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addgene 124811  (Addgene inc)


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    Structured Review

    Addgene inc addgene 124811
    Addgene 124811, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/addgene+124811/PX459-gR2A_Hinge+(Plasmid+%23124811)/pmc12270900-245-55-55
    Average 93 stars, based on 40 article reviews
    addgene 124811 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Cloning:

    Article Title: Site-directed multivalent conjugation of antibodies to ubiquitinated payloads.
    Article Snippet: .. Cloning of CRISPR-Cas9 and donor constructs The genomic sequences of the rIgG2a and mIgG2a heavy chain loci were identified via the Ensembl rate genome build Rnor_6.0 and used for the design of the different HDR donor templates. guide RNA (gRNA) for the rIgG2a constructs were previously described27: for Hinge HDR constructs, gRNA-H, GACTTACCTGTACATCCACA, Addgene 124808; for isotype switch, gRNA-ISO, TGTAGACAGCCACAGACTTG, Addgene 124811. .. For the hinge region of mIgG2a gRNA-85 (TGGAGGACAGGGCTTGATTG), gRNA-76 (GGGCTTGATTGTGGGCCCTC) and gRNA-102 (TTACCTGGGCATTTGCATGG) were designed using the CRISPR tool from the Zhang laboratory (http://crispr.mit.edu) and ordered as single-stranded oligos from Integrated DNA Technologies (IDT) with the appropriate overhangs for cloning purposes.

    CRISPR:

    Article Title: Site-directed multivalent conjugation of antibodies to ubiquitinated payloads.
    Article Snippet: .. Cloning of CRISPR-Cas9 and donor constructs The genomic sequences of the rIgG2a and mIgG2a heavy chain loci were identified via the Ensembl rate genome build Rnor_6.0 and used for the design of the different HDR donor templates. guide RNA (gRNA) for the rIgG2a constructs were previously described27: for Hinge HDR constructs, gRNA-H, GACTTACCTGTACATCCACA, Addgene 124808; for isotype switch, gRNA-ISO, TGTAGACAGCCACAGACTTG, Addgene 124811. .. For the hinge region of mIgG2a gRNA-85 (TGGAGGACAGGGCTTGATTG), gRNA-76 (GGGCTTGATTGTGGGCCCTC) and gRNA-102 (TTACCTGGGCATTTGCATGG) were designed using the CRISPR tool from the Zhang laboratory (http://crispr.mit.edu) and ordered as single-stranded oligos from Integrated DNA Technologies (IDT) with the appropriate overhangs for cloning purposes.

    Construct:

    Article Title: Site-directed multivalent conjugation of antibodies to ubiquitinated payloads.
    Article Snippet: .. Cloning of CRISPR-Cas9 and donor constructs The genomic sequences of the rIgG2a and mIgG2a heavy chain loci were identified via the Ensembl rate genome build Rnor_6.0 and used for the design of the different HDR donor templates. guide RNA (gRNA) for the rIgG2a constructs were previously described27: for Hinge HDR constructs, gRNA-H, GACTTACCTGTACATCCACA, Addgene 124808; for isotype switch, gRNA-ISO, TGTAGACAGCCACAGACTTG, Addgene 124811. .. For the hinge region of mIgG2a gRNA-85 (TGGAGGACAGGGCTTGATTG), gRNA-76 (GGGCTTGATTGTGGGCCCTC) and gRNA-102 (TTACCTGGGCATTTGCATGG) were designed using the CRISPR tool from the Zhang laboratory (http://crispr.mit.edu) and ordered as single-stranded oligos from Integrated DNA Technologies (IDT) with the appropriate overhangs for cloning purposes.

    Article Title: Site-directed multivalent conjugation of antibodies to ubiquitinated payloads
    Article Snippet: .. The genomic sequences of the rIgG2a and mIgG2a heavy chain loci were identified via the Ensembl rate genome build Rnor_6.0 and used for the design of the different HDR donor templates. guide RNA (gRNA) for the rIgG2a constructs were previously described : for Hinge HDR constructs, gRNA-H, GACTTACCTGTACATCCACA, Addgene 124808; for isotype switch, gRNA-ISO, TGTAGACAGCCACAGACTTG, Addgene 124811. .. For the hinge region of mIgG2a gRNA-85 (TGGAGGACAGGGCTTGATTG), gRNA-76 (GGGCTTGATTGTGGGCCCTC) and gRNA-102 (TTACCTGGGCATTTGCATGG) were designed using the CRISPR tool from the Zhang laboratory ( http://crispr.mit.edu ) and ordered as single-stranded oligos from Integrated DNA Technologies (IDT) with the appropriate overhangs for cloning purposes.

    Genomic Sequencing:

    Article Title: Site-directed multivalent conjugation of antibodies to ubiquitinated payloads.
    Article Snippet: .. Cloning of CRISPR-Cas9 and donor constructs The genomic sequences of the rIgG2a and mIgG2a heavy chain loci were identified via the Ensembl rate genome build Rnor_6.0 and used for the design of the different HDR donor templates. guide RNA (gRNA) for the rIgG2a constructs were previously described27: for Hinge HDR constructs, gRNA-H, GACTTACCTGTACATCCACA, Addgene 124808; for isotype switch, gRNA-ISO, TGTAGACAGCCACAGACTTG, Addgene 124811. .. For the hinge region of mIgG2a gRNA-85 (TGGAGGACAGGGCTTGATTG), gRNA-76 (GGGCTTGATTGTGGGCCCTC) and gRNA-102 (TTACCTGGGCATTTGCATGG) were designed using the CRISPR tool from the Zhang laboratory (http://crispr.mit.edu) and ordered as single-stranded oligos from Integrated DNA Technologies (IDT) with the appropriate overhangs for cloning purposes.

    Article Title: Site-directed multivalent conjugation of antibodies to ubiquitinated payloads
    Article Snippet: .. The genomic sequences of the rIgG2a and mIgG2a heavy chain loci were identified via the Ensembl rate genome build Rnor_6.0 and used for the design of the different HDR donor templates. guide RNA (gRNA) for the rIgG2a constructs were previously described : for Hinge HDR constructs, gRNA-H, GACTTACCTGTACATCCACA, Addgene 124808; for isotype switch, gRNA-ISO, TGTAGACAGCCACAGACTTG, Addgene 124811. .. For the hinge region of mIgG2a gRNA-85 (TGGAGGACAGGGCTTGATTG), gRNA-76 (GGGCTTGATTGTGGGCCCTC) and gRNA-102 (TTACCTGGGCATTTGCATGG) were designed using the CRISPR tool from the Zhang laboratory ( http://crispr.mit.edu ) and ordered as single-stranded oligos from Integrated DNA Technologies (IDT) with the appropriate overhangs for cloning purposes.



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    93
    Addgene inc addgene 124811
    Addgene 124811, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/addgene+124811/PX459-gR2A_Hinge+(Plasmid+%23124811)/pmc12270900-245-55-55
    Average 93 stars, based on 1 article reviews
    addgene 124811 - by Bioz Stars, 2026-09
    93/100 stars
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